Genotyping of <i>Entamoeba histolytica</i> by Real-Time Polymerase Chain Reaction with Sybr Green I and Melting Curve Analysis
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چکیده
منابع مشابه
Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.
Following the initial report of the use of SYBR Green I for real-time polymerase chain reaction (PCR) in 1997, little attention has been given to the development of alternative intercalating dyes for this application. This is surprising considering the reported limitations of SYBR Green I, which include limited dye stability, dye-dependent PCR inhibition, and selective detection of amplicons du...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Veterinary Medicine
سال: 1970
ISSN: 2308-0922,1729-7893
DOI: 10.3329/bjvm.v4i1.1526